Scientists have overcome a major hurdle for humans to receive life-saving organ transplants from pigs, thanks to a powerful new gene-editing technique.
Scientists have overcome a major hurdle for humans to receive life-saving organ transplants from pigs, thanks to a powerful new gene-editing technique. In a new study, the gene editing system known as "CRISPR Cas9" has been used to genetically engineer pig DNA in 62 locations - a huge leap forward in CRISPR's capability.
Executed by researchers at the Wyss Institute for Biologically Inspired Engineering at Harvard University and Harvard Medical School to inactivate retroviruses(any group of RNA viruses which insert their DNA copy into a host cell to replicate) found natively in the pig genome-complete set of DNA with genes- that has so far inhibited pig organs from being suitable for transplant in human patients. With the retroviruses safely removed via genetic engineering, however, the road is now open to the possibility that humans could one day receive life saving organ transplants from pigs, researchers said.
But why pigs? Pigs in particular have been especially promising candidates due to their similar size and physiology to humans. In fact, pig heart valves are already commonly sterilised and de cellularised for repairing or replacing human heart valves. But the transplant of whole, functional organs comprised of living cells and tissue constructs has presented a unique set of challenges for scientists. One of the primary problems has been the fact that most mammals, including pigs, contain repetitive, latent retrovirus fragments in their genomes - present in all their living cells - that are harmless to their native hosts but can cause disease in other species.
"The presence of this type of virus found in pigs known as porcine endogenous retroviruses or PERVs brought over a billion of dollars of pharmaceutical industry investments into developing xenotransplant methods to a standstill by the early 2000s," said George Church, .
Using CRISPR Cas9 like a pair of molecular scissors, George Church, Professor of Genetics at Harvard Medical School (HMS), and his team have inactivated all 62 repetitive genes in pig DNA, surpassing a significant obstacle on the path to bringing xenotransplantation to clinical reality. The study was published in the journal Science.
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