The 30-share barometer, which had gained 104.53 points in the past two sessions, declined by 21.92 points, or 0.09%, to 22,486.50 with stocks of metals, banking and IT sectors coming under some pressure.
Similarly, the NSE Nifty also traded lower by 4.25 points, or 0.06%, to 6,711.05.
Brokers said apart from profit-booking by participants at prevailing levels, a weak trend on the other Asian bourses following overnight weakness at the US market on disappointing corporate earnings mainly dampened the trading sentiments here.
Among other Asian markets, Japan's Nikkei fell by 2.30%, while Hong Kong's Hang Seng shed 0.33% in early trade on Wednesday. The US Dow Jones Industrial Average ended 0.78% lower in on Tuesday's trade.